Figure 4.

SAXS analysis of Vps75 under tetrameric and dimeric conditions. (A) Normalized pair distance distribution functions (r) for Vps75 at 150 mM (blue, tetramer) and 500 mM NaCl (red, dimer). (B) Comparison of experimental SAXS curve (grey) with a CRYSOL calculated scattering curve (red) derived from a crystal structure of the dimer (PDB: 2ZD7), under high ionic conditions (500 mM sodium chloride). The crystal structure with unresolved residues modelled as a random coil is shown in the inset. (C) Comparison of the experimental SAXS curve (grey) under 150 mM sodium chloride, with a CRYSOL calculated curve of the Vps75 tetramer model (blue). The inset shows the modelled Vps75 tetramer with unresolved residues added in a random coil conformation. One dimer is shown in green, the other in blue. χ² values for each fit are shown.