Table 1. The effect of homopolymeric tract on β-gal expression.
β-gal activity (Miller units) | |||
---|---|---|---|
Out-of-frame (+1) | Out-of-frame (−1) | In-frame | |
A7 | 7 (1) | 17 (1) | 2077 (167) |
A8 | 22 (1) | 180 (16) | 1616 (134) |
A9 | 180 (15) | 390 (86) | 1260 (43) |
A10 | 308 (19) | 624 (37) | 1113 (69) |
A11 | 303 (28) | 539 (46) | 822 (55) |
U9 | 145 (10) | 307 (28) | 1652 (95) |
U10 | 431 (33) | 323 (17) | 791 (35) |
C10 | 9 (1) | 15 (9) | 1246 (76) |
G10 | 7 (4) | 10 (3) | 1647 (152) |
A5GA4 | 8 (2) | 7 (1) | 1746 (101) |
A5UA4 | 3 (1) | 4 (1) | 1698 (100) |
Average β-gal activities are presented in Miller units (bold), with standard error of the mean (SEM) in parentheses, n = ≥3.