Figure 4.

The effect of transposon-end mutations near the sites of cleavage. The kinetics of transposition reactions were analyzed with variants of a supercoiled transposon donor that carried symmetrical point mutations on both transposon ends. The nomenclature is as follows: negative numberings are for positions within the flanking DNA and positive numberings are for positions within the transposon end, as indicated in Figure 1C. A mutation is named after the nucleotide on the NTS. The products of the reactions are as illustrated in Figure 1A and described in the corresponding figure legend.