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. 2014 Mar 12;42(9):5532–5542. doi: 10.1093/nar/gku205

Figure 4.

Figure 4.

The deposition H3–H4 on DNA as a tetramer reduces the propensity of heterotypic Cse4–H3–H42 tetrasome formation. (A) 1 μM refolded (H3–H4)2 tetramer labeled with Alexa-488 was mixed with an equimolar concentration of Atto-647N labeled (H3–H4)2 tetramer (in presence or absence of 4 μM Nap1, calculated as a monomer) or Scm3–Cse4ΔN–H4 and incubated with 5 μM 79 bp DNA. Samples were incubated for 1 h at 25°C, then analyzed on a 6% native gel and scanned for fluorescence as in Figure 2A. (B) Tetrasomes assembled on 79 bp DNA as in (A), were analyzed on 6% native gel and stained with ethidium bromide. The band indicated by (*) is a heterotypic tetramer.