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RECQL1 and WRN may act in the same pathway. (A) Double depletion of both RECQL1 and WRN was performed in U2OS cells and analyzed for telomere lengths by using flow-FISH analysis. (B) Western blot showing double depletion of RECQL1 (by using siRNA) and WRN (using shRNA). (C) Telomere length analysis was further studied by performing flow-FISH coupled with cell cycle analysis on stable KD cells of RECQL1 or WRN, and the western blots indicating the knockdown efficiency were shown below.
