Figure 2.

Determination of the RNA-binding capacity of Gemin5 truncated polypeptides. (A) Gel-shift assay conducted with increasing amounts of purified His-tagged Gemin5 polypeptides depicted at the top and radiolabelled domain 5 RNA, fractionated in native gels and visualized by autoradiography. (B) Gemin5 C-terminal region structure prediction. Jpred3 program (Jnet version: 2.2, www.compbio.dundee.ac.uk) was used for the secondary structure prediction analysis. Helical prediction scores below 7 are shown in light grey and scores between 8 and 9 in dark grey. High scores represent higher probability of adopting a helical conformation. Coil-coiled regions are displayed as a grey thick line and labelled in white Cs. Blue and brown boxes represent the boundaries of the two regions analyzed; dark brown box depicts the overlapping sequence.