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. 2014 Mar 5;42(9):5742–5754. doi: 10.1093/nar/gku177

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© The Author(s) 2014. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Differential effect of Gemin5 C-terminal truncated polypeptides on IRES activity. (A) Diagram of the silencing and co-transfection assay with indication of harvesting time (h), depending on the expressed Gemin5 construct. Effect of expression of G5_1287–1508 (B), G5_1297–1412 (C) and G5_1383–1508 (D) on IRES-dependent translation (striped bars) monitor by LUC activity, relative to the values observed in control siRNA treated cells (empty bars). Black bars depict the effect of Gemin5 silencing on IRES-dependent translation. Values represent the mean ± SD (***P < 0.005; **P < 0.01). A Gemin5 WB (top panel) shows the silencing (Gemin5, p170 band) and the expression of the indicated G5 construct separated on 15% SDS-PAGE. Tubulin is used as loading control. Mobility of MW markers is indicated at the left of each WB.