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. 2014 Mar 20;42(9):5894–5906. doi: 10.1093/nar/gku222

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — (A) Diagrams of hilD mRNA and hilD Δ3′UTR mRNA. The thick line in the 3′ region of hilD Δ3′UTR mRNA represents the 82-nt pKD13 scar. The Rho-independent transcriptional terminator is left intact in the constructs. (B and C) Northern blots of hilD and hilD Δ3′UTR mRNAs when hilD is transcribed from its own promoter (B), and when transcription is driven by the P_LtetO promoter (C). Three discrete bands are detected with a riboprobe when hilD is transcribed from its native promoter. rnpB mRNA has been used as loading control. For quantification, the ratio hilD/rnpB was normalized to 1 in hilD mRNA carrying a native 3′UTR.