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. 2014 Mar 20;42(9):5894–5906. doi: 10.1093/nar/gku222

Figure 4.

Figure 4.

(A) Western blots of InvF-3xFLAG, InvH-3xFLAG, SipB-3xFLAG and SipC-3xFLAG in protein extracts from hilD 3′UTR+ and hilD 3′UTR− hosts. GroEL was used as loading control. For quantification, the ratio 3xFLAG-tagged protein/GroEL was relativized to 1 in the hilD 3′UTR+ background. (B) β-Galactosidase activity of a sipC::lac fusion in Salmonella enterica strains containing a native hilD 3′UTR (wt) or alleles with deletions of the hilD 3′UTR (Δ3′UTR, 3′UTR200, 3′UTR150, 3′UTR100, 3′UTR50, 3′UTR25 and 3′UTR50end).