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. 2014 Apr 17;42(9):5556–5566. doi: 10.1093/nar/gku220

Figure 4.

Figure 4.

Role of MYC2 in the memory behavior of [+/−] ‘revised-response’ memory genes. (A) Transcript levels of MYC2 in wild type, myc2 and 35S:: MYC2 backgrounds. (B and C) Transcript levels of the six ABA-dependent, AREB1/AREB2/ABF3-independent [+/−] memory genes (see Figure 3, above) analyzed in wild type, myc2 mutant and MYC2-overexpressing backgrounds illustrating all six [+/−] genes require MYC2 for optimal expression in S1; (B) [+/−] ‘revised-response’ memory genes that have lost memory responses in MYC2-overexpressing background, displaying high transcript levels in all four treatment states in the absence of stress; (C) [+/−] ‘revised-response’ memory genes that depend on MYC2 for expression in S1 but are not affected by the constitutive presence of MYC2 in the overexpressing background. (D) The signature MYB2-dependent gene, RD22, is induced in S2, despite low MYB2 levels in wild type. Overexpression of MYB2 raises RD22 transcription irrespective of stress signals. UBQ10 was used as an internal control. Experiments were repeated three times, each with three replicates, and the representative experiment shown indicates the mean ± SEM, n = 3 replicates. Asterisks indicate statistical significance between S1 and S3 in wild type, myc2 mutant and MYC2 overexpressing lines, respectively. Statistical analysis was performed based on Student's t-test. *P < 0.01.