Table 1. RNA sequences used in the study and binding parameters to Toscana virus N.
| Oligo | Sequence | Binding affinity | Stoichiometry |
|---|---|---|---|
| Oligo1 (used for crystallization) | UGUGUUUCU | 260 nM ± 20 nM | 0.49 ± 0.01 |
| Oligo2 (3′ genomic consensus sequence) | UCUUUGUGU | 560 nM ± 70 nM | 0.43 ± 0.01 |
| Oligo3 (5′ genomic consensus sequence) | ACACAGAGA | not determined | not determined |
| Oligo4 (G2A mutant of oligo1) | UAUGUUUCU | 290 nM ± 40 nM | 0.49 ± 0.01 |
| Oligo5 (polyU 9mer) | UUUUUUUUU | 360 nM ± 40 nM | 0.40 ± 0.01 |
| Oligo6 (8-mer RNA derived from oligo1) | UGUGUUUC | 710 nM ± 30 nM | 0.51 ± 0.01 |
| Oligo7 (7-mer RNA derived from oligo1) | UGUGUUU | 2.7 μM ± 0.2 μM | 0.52 ± 0.01 |
| Oligo8 (6-mer RNA derived from oligo1) | UGUGUU | 8.0 μM ± 0.9 μM | 0.50 ± 0.03 |
| Y32A + oligo1 | UGUGUUUCU | 1.6 μM ± 0.2 μM | 0.46 ± 0.01 |
| K79A + oligo 1 | UGUGUUUCU | 220 nM ± 30 nM | 0.42 ± 0.01 |
| Y32A–K79A + oligo 1 | UGUGUUUCU | 6 μM ± 2 μM | 0.43 ± 0.05 |
| K204A + oligo1 | UGUGUUUCU | 250 nM ± 10 nM | 0.41 ± 0.01 |
| RVFV N +oligo 1 | UGUGUUUCU | 400 nM ± 100 nM | 0.70 ± 0.02 |