Figure 3.

Functional analyses of YqeY and the helical domain of GatB. (A) Model of the GatFAB-tRNA complex. tRNA^Asn from T. thermophilus (PDB ID: 3KFU) and the helical and YqeY domains of GatB from T. maritima (PDB ID: 3AL0) are docked onto the GatFABΔHY structure. The modeled tRNA^Asn and the C-terminal domains of GatB are colored yellow and cyan, respectively. GatFAB is colored as in Figure 1. (B) Growth phenotypes of GatB mutants on fermentative (SC Glucose) or respiratory (SC Glycerol) media. (C) Bioimager scan of the TLC plate for a transamidase assay, which shows the absence of conversion of H. pylori tRNA^Gln-bound [¹⁴C]Glu into [¹⁴C]Gln, catalyzed by the GatFAB mutants with the C-terminal deletions of GatB (ΔY and ΔHY).