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. 2014 Apr 1;42(9):6052–6063. doi: 10.1093/nar/gku234

Figure 5.

Figure 5.

Functional analyses of the GatF NTD. (A) Secondary structures of GatF mutants are shown (left). Growth phenotypes of GatF mutants on fermentative (SC Glucose) or respiratory (SC Glycerol) media (right). (B) Quantification of the activities of the GatFABF37–183 and GatFABF47–183 mutants relative to the full-length GatFAB. Glutaminase activity as well as transamidase activities with glutamine or NH4Cl as the amide donor are shown for GatF mutants relative to the activity of the full-length GatFAB. Results are the average of three experiments, with error bars representing standard deviation (See Supplementary Figure S5).

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