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. 2014 Mar 20;42(9):5830–5845. doi: 10.1093/nar/gku214

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Primase activity of human PrimPol. (A) Human PrimPol has primase activity and can produce de novo primers using rNTPs and dNTPs opposite a poly(dT) template. (B) PrimPol_ZF-KO lacks de novo primer synthesis activity, suggesting that an intact zinc finger is required for primase activity. (C) PrimPol_1–487 also has primase activity similar to the wild-type PrimPol. The unstructured region that is downstream of the zinc finger is therefore not required for primase activity. (D) PrimPol_1–354 has no primase activity, which indicates that PrimPol requires a functional zinc finger for primer synthesis.