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. 2013 Oct 15;2(10):e128. doi: 10.1038/mtna.2013.52

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Copyright © 2013 American Society of Gene & Cell Therapy

Molecular Therapy-Nucleic Acids is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivative Works 3.0 License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Effect of injection volume on hydrodynamic gene delivery efficiency. (a) The balloon catheter was inserted into the right lateral hepatic vein (RLHV), (b) right medial hepatic vein (RMHV), or (c) left lateral hepatic vein (LLHV) followed by the venography. Each lobe was injected from the intermediate site with the volume of 1.25×, 2.5×, or 3.0× lobe volume. (d) Level of luciferase gene expression in right lateral lobe (RLL), (e) right medial lobe (RML), (f) and left lateral lobe (LLL). Two lobes from two different animals were used for each volume and 15 liver samples from different parts of each targeted lobe were used for luciferase assay (n = 30 for each data point). The values represent mean ± SD. *P < 0.05, one-way ANOVA followed by Bonferroni's multiple comparison test.