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. 2013 Oct 15;2(10):e128. doi: 10.1038/mtna.2013.52

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Copyright © 2013 American Society of Gene & Cell Therapy

Molecular Therapy-Nucleic Acids is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivative Works 3.0 License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Tissue distribution of plasmid DNA. Multiplex PCR analysis was performed on genomic DNA collected from pigs by 35 cycles of PCR amplification using primers for ampicillin resistance (Amp) and glyceraldehyde 3-phosphate dehydrogenase (Gapdh) genes, respectively. Lanes: 1, plasmid DNA; 2, normal liver without plasmid injection; 3, injected lobe in pig 2; 4, spleen; 5, kidney; 6, brain; 7, heart; 8, stomach; 9, colon; 10, ovary; 11, lung. The arrow represents the 660 bp Amp fragment. The arrowhead represents the 122 bp Gapdh fragment.