. 2013 Oct 30;4(12):1249. doi: 10.1021/ml4004167

Table 1. pK_a Values of Monoacidic, Monobasic, and Dibasic Compounds Determined by the 96-Well UV Spectrophotometric Method.

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The use of 2% v/v DMSO as cosolvent did not alter significantly the pK_a value of the test compounds. Working temperature = 30 °C. All pK_a were measured at constant ionic strength (I = 0.1 M) and concentration (C = 0.2 mM).

Analytical wavelengths are determined at the maximum and minimum absorption values in the spectral difference plot.

Experiments were repeated at least three times.

Standard deviation.

Experimental pK_a values at 25 °C in water.

Table 1. pKa Values of Monoacidic, Monobasic, and Dibasic Compounds Determined by the 96-Well UV Spectrophotometric Method.

Table 1. pK_a Values of Monoacidic, Monobasic, and Dibasic Compounds Determined by the 96-Well UV Spectrophotometric Method.