Caspase 3/7 activity
was measured in 3E3 cells (A, ectopically
expressing autotaxin) or 3V5 cells (B, transfected with vector) following
incubation with 10 μM compound 4, 300 nM S32826,
with or without 300 μM carboplatin for 18 h. Caspase 3/7 activity
was normalized for cell number measured by staining with SRB. The
results (mean ± S.D., n = 3–5) differed
significantly from cells treated with LPC and carboplatin where shown:
* P < 0.05, ** P < 0.01, *** P < 0.001 vs (one-way anova). RLU, relative luminescence
units.