Figure 2.

The regulation of both BMI-1 and CD133 in OSCC cells. (A) The Id1 and NF-κB subunit p65 were expressed in the clinical OSCC tissue sections. (B) RT-PCR demonstrated that both CD133 and BMI-1 mRNA transcripts were faintly detected in OSCC tissue cultures. (C) In cultured CA9-22 cells, BMI-1 was undetected in controls but detected in both Id1 and p65 transfected cells (3 days after transfection). CD133 was not detected in controls but detected in Id1 and p65 transfected cells, obsviously upregulated by both Id1+p65 (IP). (D) IP synergistically increased the percentage of BMI-1⁺ cells, but not Id1 and p65 alone by FACS in OSCC tissue cultures. ^*p<0.05.