Figure 3.

The relationship between Id1⁺ and BMI-1⁺ cells as well as Id1⁺ and BrdU+ in OSCC cells. Cells were stained with both Id1 and BMI-1 antibodies and examined by FACS. (A) In OSCC cells, as indicated by marker R11, there were 3.4% of Id1⁺ cells. (B) Approximately 97.6% of total cells were Id1⁻ and these cells were also BMI-1⁻. (C) Among Id1⁺ cells, 99.8% were BMI-1⁺. (D) In a similar way, as measured by marker R14, there were approximately 3% of BMI-1⁺ cells. (E) Among BMI-1⁻ cells, accounting for 97% of total cells, were also Id1⁻. (F) Among the Id1⁺ cells, they were 81.3% BMI-1⁺. In the Id1⁺ subpopulation, there were 82.8% of cells remaining Id1⁺ after culture for 24 h (G). In the Id⁻ cells, 52.2% of cells remained BrdU⁺ (H) whereas in the Id1⁺ cells 98.6% of cells remained BrdU⁺ (I). Accordingly, in the Id1⁺ cell cultures, there were 96.3% of BrdU⁺ cells (J). In the BrdU⁻ cells 8.1% of cells were Id1⁺ (K) whereas in the BrdU⁺ cells 79.0% of cells were Id1⁺ (L). Transfection with ev, Id1, p65 and IP indicated that Id1, p65, and IP significantly increased the cell cycle progression (M), cell counts (N), and BrdU incorporation (O) of CA9-22 cells. ^*p<0.05.