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. 2014 Mar 5;44(5):1425–1432. doi: 10.3892/ijo.2014.2326

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Copyright © 2014, Spandidos Publications

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Figure 1. — RT-PCR analysis of TSGCT. (A) cDNA fragment amplification using the primer set CSF1-1886F/S100A10-840R. (B) cDNA amplification of the wild-type S100A10 using the primer set S100A10-555F/S100A10-840R. (C) cDNA amplification of the CSF1 transcript 1 using the primer set CSF1-1886F/CSF1-2176R. (D) cDNA amplification of the CSF1 transcript 4 using the primer set CSF1-1886F/CSF1-trans42130R. Lane M, 1 kb Plus DNA ladder (GeneRuler, Fermentas); lane 1, cDNA from case 1; lane 2, cDNA from case 2; lane 3, cDNA from case 3; lane 4, cDNA synthesized from Human Universal Reference Total RNA (Takara); lane 5, blank, no RNA in cDNA synthesis. (E) Partial sequence chromatogram of the cDNA fragment amplified with the primer set CSF1-1886F/S100A10-840R showing the fusion of exon 8 of CSF1 with exon 3 of S100A10. The stop codon TAG is in the box.