Table II.
Standard curve analyses of the real-time PCR for the quantification of the expression of CSF1 transcripts 1, 4 and 5, and CSF1-S100A10.
| Template (purified PCR) product | Gene-transcript | Accession no. | Primer set for real-time PCR | Efficiency (E %) | Correlation coefficient (R^2) | Slope | y-intercept |
|---|---|---|---|---|---|---|---|
| CSF1-1886F/CSF1-2176R | CSF1-transcript 1 | NM_000757 version 5 | CSF1-2057F/CSF1-trans1-2123R | 107.1 | 0.998 | 3.164 | 29.532 |
| CSF1-1886F/CSF1-trans4-2130R | CSF1-transcript 4 | NM_172212 version 2 | CSF1-2057F/CSF1-trans4-2120R | 98.3 | 0.997 | 3.364 | 29.690 |
| CSF1-1886F/CSF1-3end-R1out | CSF1-transcript 5 | - | CSF1-2057F/CSF1-transNew-Rq | 97.6 | 0.997 | 3.380 | 30.662 |
| CSF1-1886F/S100A10-840R | CSF1-S100A10 | - | CSF1-2057F/S100A10-664R | 110.1 | 0.991 | 3.102 | 29.282 |