Figure 5.

TRAIL and sulforaphane eliminate primary prostate CSCs synergistically via the induction of apoptosis and inhibition of proliferation and CSC marker expression. (A) Primary prostate CSCs were treated as described in Fig. 1A. Twenty-four hours after TRAIL treatment, the cells were cytospinned onto glass slides, and immunohistochemistry staining for proliferation (Ki67), apoptosis (cleaved fragment of active caspase-3), and CSC markers (CD133, CXCR4, c-Met, CD44, EpCAM and SOX2) was performed and visualized using microscopy. Representative images are shown and the arrows indicate positive cells. The bar indicates 25 μm. (B) The number of positive cells after each staining procedure was quantified in 10 vision fields under ×400 magnification, and the means ± SD are shown. ^**p<0.01.