Table I.
CSC characteristics of established human AIPC cell lines.
| DU145 | PC3 | Ref(s). | |
|---|---|---|---|
| ATCC no. | HTB-81 | CRL-1435 | ATCC |
| Source | Brain metastasis | Bone metastasis, grade IV | ATCC |
| p53 status | Mutant | Absent | (14) |
| Growth characteristics | Adherent | Adherent | ATCC |
| Colony-forming capacity | High | High | (15) |
| Spheroid-formation capacity | None | None | (9) |
| ALDH activity | 2.4±0.3 | 6.3±1.0 | (16) |
| Growth in nude mice | Rapid, 100 CD44+/CD24− cells form a tumor in 5 of 5 mice within 80 days | Rapid, 100 PC3 cells form a tumor in 1 of 8 mice within 90 days | (17,18) |
| CD44+/CD24− | 7–10% | Detectable | (9,17,19,20) |
| CD133 | 0.01% | Setectable | (15,21) |
| α2β1 | 1–10% | 100% | (22) |
| Invasiveness in Matrigel | > 20%, highly invasive | Highly invasive | (23,24) |
| E-cadherin expression | Low | Low | (23,25) |