Table 1.
dlx3b/dlx4b MO effects in zebrafisha
| Injection | Amounts (ng/embryo) |
N | Phenotype (reduced or absent) | Defective gastrulationc |
Deadc | |
|---|---|---|---|---|---|---|
| RBs | TGb | |||||
| Control | 40 | 38 | 0 | 0 | – | – |
| MO | 108 | – | – | 0 | 7 | |
| dlx3bhd– | 0.150–0.200 | 40 | 18 | – | ND | ND |
| EnR | 44 | – | 14 | |||
| dlx3b/dlx4b | 40 | 109 | 52 | – | – | – |
| ATG– | 73 | – | 39 | – | – | |
| MO | 273 | – | – | 17 | 22 | |
| dlx3b/dlx4b | 40 | 56 | 32 | – | – | – |
| E2I2– | 51 | – | 38 | – | – | |
| MO | 97 | – | – | 5 | 11 | |
| dlx3b/dlx4b | 40 | |||||
| ATG– | 30 | 4d | ND | ND | ND | |
| MO+ | 0.150 | |||||
| dlx3bhd– | ||||||
| VP16 | ||||||
ND is not done.
a
All sets of injection experiments were done at least three times.
b
RB = Rohon–Beard sensory neuron, TG = Trigeminal ganglia placode.
c
Checked at the 3-somite stage.
d
We have defined reduced to have 12 or less RB neurons/per embryo. This is the average number of total RB neurons in the MO-injected embryos (see Table 3).