Table 2.
Quantitative trait locus analysis of metabolic syndrome-related phenotypes and atherosclerotic lesion area in [(PERAxB6-Ldlr−/−)F1 × B6-Ldlr−/−]N2 mice. Only significant loci with genome-wide Pemp ≤ 0.05 are presented.
| Linked marker | Chr Position (cM)* | Trait | LOD (%VAR)† males (n=75) | LOD (%VAR) females (n=92) | LOD (%VAR) combined (n=167) | Pemp‡ | Genotype associated with higher value (% increase)§ |
|---|---|---|---|---|---|---|---|
| D1Mit270 | Chr 1 (92) | Insulin|| | --¶ | 2.9 (16%) | -- | ≤0.02 | BP (127%) |
| D2Mit405 | Chr 2 (69) | Lesion area|| | 2.8 (16%) | 1.1 (6%) | 3.9 (11%) | ≤0.04 | BB (27%) |
| D4Mit143 | Chr 4 (43) | TG†† | 3.6 (20%) | 1.2 (6%) | 4.8 (13%) | ≤0.004 | BP (52%) |
| BWT** | 2.0 (11%) | 2.0 (10%) | 4.0 (11%) | ≤0.05 | BP (10%) | ||
| D7Mit253 | Chr 7 (53) | HDL-C†† | 3.1 (18%) | --¶ | -- | ≤0.04 | BB (26%) |
| D8Mit291 | Chr 8 (16) | BWT** | 3.4 (19%) | --¶ | -- | ≤0.02 | BB (15%) |
| D13Mit134 | Chr 13 (6) | BWT†† | 2.9 (16%) | --¶ | -- | ≤0.04 | BP (14%) |
| D13Mit139 | Chr 13 (32) | HDL-C†† | 4.6 (26%) | --¶ | -- | <0.001 | BP (30%) |
| D13Mit144 | Chr 13 (48) | Insulin†† | 1.1 (7%) | 3.2 (15%) | 4.3 (11%) | ≤0.02 | BP (53%) |
BB, homozygous for B6 alleles; BP, heterozygous for B6 and PERA/Ei alleles.
Chr position indicates the distance of the peak marker from the centromere in centiMorgans (cM) as listed in the Mouse Genome Database (http://informatics.jax.org/)
LOD, logarithm of odds ratio score; %VAR, percent variance explained by the locus
Pemp, empirically-determined probability for observing the corresponding LOD score by chance (calculated using the permutation test function of Map Manager QTXb19)
for traits linked in both sexes, the average percent increase for males and females is reported
after feeding a Western-type diet for 3 months; mice were 20 weeks of age
indicates LOD < 1
after feeding a Western-type diet for 2 weeks; mice were 10 weeks of age
after feeding a laboratory chow diet; mice were 8 weeks of age