Table 2.

The intra-day (n = 5) and inter-day (n = 15) precision (RSD) and accuracy (RE) of the LC–MS/MS method used to quantitate COT, NCOT, OHCOT and COTNO in rat plasma and brain homogenate.

			Intra-day			Inter-day
	Analyte	Nominal Conc. (ng/mL)	Measured Conc. (ng/mL)	RSD (%)	RE (%)	Measured Conc. (ng/mL)	RSD (%)	RE (%)
Plasma	COT	1.0	0.92±0.11	12.30	−8.00	0.91±0.12	13.44	−9.27
		3.0	3.08±0.31	10.03	2.67	2.87±0.26	9.12	−4.42
		30.0	30.05±0.89	2.97	0.15	28.63±1.25	4.37	−4.56
		75.0	74.79±1.73	2.31	−0.28	71.86±2.58	3.59	−4.18
	NCOT	1.0	0.92±0.03	3.44	−8.00	0.92±0.06	6.13	−8.33
		3.0	2.99±0.05	1.71	−0.40	2.89±0.11	3.85	−3.76
		30.0	29.49±0.44	1.48	−1.71	28.22±1.05	3.71	−5.92
		75.0	72.05±1.06	1.47	−3.93	69.93±2.31	3.30	−6.76
	OHCOT	1.0	0.87±0.02	2.21	−12.80	0.91±0.07	7.49	−8.80
		3.0	2.83±0.08	2.93	−5.53	2.81±0.09	3.22	−6.40
		30.0	28.36±0.64	2.26	−5.47	28.38±0.49	1.71	−5.39
		75.0	70.79±1.12	1.59	−5.61	70.70±1.20	1.70	−5.73
	COTNO	1.0	0.904±0.06	6.71	−9.60	0.90±0.17	18.85	−10.20
		3.0	2.886±0.20	6.84	−3.80	2.87±0.24	8.41	−4.36
		30.0	27.234±1.18	4.34	−9.22	27.16±0.85	3.12	−9.48
		75.0	66.53±1.26	1.89	−11.29	67.77±3.22	4.75	−9.63
Brain	COT	1.0	0.94±0.08	9.00	−6.00	0.90±0.10	10.72	−9.53
		30	2.82±0.04	1.55	−6.07	2.82±0.07	2.52	−6.02
		30.0	27.12±0.52	1.91	−9.59	27.94±0.83	2.98	−6.86
		75.0	70.14±1.47	2.09	−6.48	72.05±2.12	2.94	−3.93
	NCOT	1.0	0.94±0.03	2.81	−6.00	0.94±0.04	4.55	−6.27
		3.0	2.90±0.05	1.60	−3.33	2.94±0.08	2.59	−2.00
		30.0	27.99±0.88	3.15	−6.71	28.52±1.11	3.89	−4.94
		75.0	71.66±3.62	5.05	−4.45	72.71±3.23	4.45	−3.05
	OHCOT	1.0	0.92±0.03	3.64	−7.60	0.90±0.04	4.35	−10.13
		3.0	2.92±0.08	2.66	−2.53	2.92±0.06	2.21	−2.53
		30.0	28.63±0.72	2.51	−4.57	28.67±0.53	1.85	−4.44
		75.0	72.64±3.40	4.68	−3.15	72.89±2.08	2.86	−2.82
	COTNO	1.0	1.01±0.05	4.73	0.80	1.03±0.15	14.73	2.60
		3.0	3.00±0.11	3.77	−0.07	2.97±0.14	4.57	−0.84
		30.0	29.61±0.22	0.75	−1.31	27.86±1.44	5.17	−7.14
		75.0	75.65±4.57	6.04	0.87	72.08±4.01	5.56	−3.89