Table 3.

Absolute recovery (%AR), relative recovery (%RR) and matrix effect (%ME) of the method. Mean ± SD values are shown for all the metabolites at 3.0, 30.0 and 75.0 ng/mL concentrations in plasma and brain homogenate. Types of matrix effect are shown in percentage of enhancement or suppression.

Matrix	Analyte	Conc. (ng/ml)	AR (%)	RR (%)	ME (%)	Type
Plasma	COT	3.0	37.26±1.20	52.94±1.71	70.38	29.62% suppression
		30.0	23.82±8.41	40.54±14.31	58.75	41.25% suppression
		75.0	30.79±7.42	41.97±10.12	73.36	26.64% suppression
	NCOT	3.0	62.42±5.66	76.80±6.97	81.28	18.72% suppression
		30.0	63.41±9.13	83.90±12.09	75.57	24.43% suppression
		75.0	66.51±8.04	73.55±8.89	90.43	9.57% suppression
	OHCOT	3.0	36.04±2.32	89.51±5.75	40.26	59.74% suppression
		30.0	37.09±12.42	73.61±24.66	50.38	49.62% suppression
		75.0	41.56±3.49	76.77±6.45	54.14	45.86% suppression
	COTNO	3.0	33.54±9.73	41.81±12.14	80.22	19.78% suppression
		30.0	45.83±2.21	56.99±2.75	80.41	19.59% suppression
		75.0	34.56±4.00	45.06±5.21	76.69	23.31% suppression
Brain	COT	3.0	41.27±4.39	58.75±6.24	70.25	29.75% suppression
		30.0	30.88±2.73	37.60±3.33	82.12	17.88% suppression
		75.0	39.15±3.38	63.01±5.43	62.12	37.88% suppression
	NCOT	3.0	60.35±1.79	75.38±2.23	80.07	19.93% suppression
		30.0	65.68±1.35	96.80±2.00	67.85	32.15% suppression
		75.0	71.84±4.98	97.27±6.75	73.86	26.14% suppression
	OHCOT	3.0	27.50±1.82	78.61±5.19	34.98	65.02% suppression
		30.0	41.30±2.91	82.95±5.85	49.79	50.21% suppression
		75.0	36.45±1.23	100.03±3.36	36.44	63.56% suppression
	COTNO	3.0	19.96±5.08	31.88±8.11	62.61	37.39% suppression
		30.0	39.24±6.20	68.00±10.74	57.71	42.29% suppression
		75.0	25.30±7.23	39.69±11.35	63.74	36.26% suppression