Table 4.
Autosampler stability (n = 5), bench-top stability (n = 3) and freeze-thaw stability (n = 3) of COT, NCOT, OHCOT and COTNO at 3.0 and 75.0 ng/mL concentrations in plasma and brain homogenate. Stabilities are shown in forms of percentage of relative concentration to time zero control (mean ± SD).
| Matrix | Analyte | Conc. (ng/mL) | Autosampler Stability (%) | Bench-top Stability (%) | Freeze-Thaw Stability (%) |
|---|---|---|---|---|---|
|
| |||||
| Plasma | COT | 3.0 | 92.36±4.57 | 97.43±8.31 | 104.10±4.59 |
| 75.0 | 98.08±3.28 | 100.15±4.88 | 102.29±1.63 | ||
|
| |||||
| NCOT | 3.0 | 98.65±3.63 | 102.65±5.47 | 99.56±2.55 | |
| 75.0 | 96.94±3.03 | 99.76±3.57 | 102.49±5.04 | ||
|
| |||||
| OHCOT | 3.0 | 100.20±2.39 | 100.82±3.47 | 97.26±1.79 | |
| 75.0 | 101.39±1.75 | 99.28±4.76 | 102.46±0.88 | ||
|
| |||||
| COTNO | 3.0 | 99.87±4.88 | 105.72±5.02 | 98.32±1.15 | |
| 75.0 | 100.54±3.19 | 101.17±4.75 | 100.31±1.58 | ||
|
| |||||
| Brain | COT | 3.0 | 101.60±1.80 | 95.26±3.94 | 95.61±10.62 |
| 75.0 | 96.95±2.38 | 102.39±6.13 | 101.88±2.98 | ||
|
| |||||
| NCOT | 3.0 | 101.53±3.28 | 100.49±3.65 | 97.09±9.24 | |
| 75.0 | 98.93±2.69 | 100.64±4.54 | 97.88±0.29 | ||
|
| |||||
| OHCOT | 3.0 | 101.74±3.81 | 100.78±5.88 | 93.70±7.34 | |
| 75.0 | 101.33±3.12 | 101.48±3.08 | 99.14±2.35 | ||
|
| |||||
| COTNO | 3.0 | 99.44±3.73 | 99.68±4.99 | 103.13±6.72 | |
| 75.0 | 98.27±2.89 | 106.59±3.08 | 95.69±2.89 | ||