Table 6.

Plasma and brain concentrations (mean ± SD) of COT, NCOT, OHCOT and COTNO in biological samples obtained from rats (n = 3) subcutaneously dosed by 1 mg/kg COT.

Analyte	Plasma Conc. (ng/ml)	Brain Conc. (ng/g)	Brain-to-Plasma Ratio***
COT	1364.35±61.58*	959.4±73.84*	0.70
NCOT	0.89±0.09**	0.48±0.06**	0.54
OHCOT	5.31±1.13	0.91±0.17**	0.17
COTNO	7.13±1.95	0.18±0.16**	0.03

^*Samples over the ULOQ were diluted 15 folds and analyzed with the method.

^**Concentrations below the LLOQ but still detectable were calculated with extrapolated calibration curves.

^***Brain-to-plasma ratios were calculated with the assumption that 1 g of brain tissue was equivalent to 1 mL of plasma.