Figure 1.

Schematic representation of pCR3.1-TALE_Frat. (a) The TALE targeting the frataxin promoter is placed under the control of the cytomegalovirus (CMV) promoter in the pCR3.1-TALE_Frat–VP64 plasmid. The TALE gene is fused with a nuclear localization signal (NLS) and the VP64 transcription activation domain (TAD). (b) A StuI restriction site was introduced in the pCR3.1-TALE_Frat–p65 plasmid before the TAD and an XbaI site after the TAD. (c) The amino acid (aa) sequence of the TALE_Frat#8-VP64 protein is presented. The repeat variable di-residues are over-lighted in gray. The VP64 sequence is included in a box.