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. 2013 Sep 10;2(9):e121. doi: 10.1038/mtna.2013.45

Figure 1.

Figure 1

Silencing of Bcl-2 by a specific siRNA inhibits proliferation and colony formation of ER(−) breast cancer cells. (a) MDA-MB-231 cells were treated with control or Bcl-2 siRNA for 48 hours and analyzed using anti-Bcl-2 monoclonal antibody by western blot analysis. (b) Silencing of Bcl-2 by siRNA inhibits size and number of colonies formed by MDA-MB-231 cells. Cells were treated with Bcl-2 or control siRNA once a week and colonies were detected 2 weeks later. Bcl-2 silencing significantly reduced colony size and area (88%, P < 0.0049) (c) and the colony number (69%, P < 0.006) (d) of MDA-MB-231 colonies as compared with nonsilencing control siRNA-treated cells (*P < 0.05). (e) Morphological appearance of breast cancer cells treated with Bcl-2 siRNA by phase contrast microscopy (72 hour-MCF7) at 10 and 40× magnification.