. Author manuscript; available in PMC: 2014 Jul 1.

Published in final edited form as: Nat Protoc. 2013 Dec 19;9(1):146–155. doi: 10.1038/nprot.2014.001

TABLE 1.

Available Spinach-based sensors.

Sensor	Ligand	EC₅₀ for ligand (μM)	K_D (nM)	Fold activation	Tested in bacteria
Ade sensor^a	Adenosine	44		16	No
ADP sensor^a	Adenosine diphosphate	270		18	Yes
Gua sensor^a	Guanine	1.5		32	No
GTP sensor^a	Guanosine triphosphate	7.7 × 10³		15	No
SAM sensor^a	S-adenosylmethionine	120		25	Yes
Vc2-Spinach^b	c-di-GMP		230	ND	Yes
G20A Vc2-Spinach^b	c-di-GMP		1,000	ND	Yes
	Camp-GMP		4,200	ND	Yes
SP_2^c	c-di-GMP	ND		ND	No
Streptavidin sensor^d	Streptavidin	ND		10	Yes
Thrombin sensor^d	Thrombin	ND		7	No
MCP sensor^d	MS2 coat protein	ND		42	Yes

Values obtained from ref. 2.

Values obtained from ref. 10.

Values obtained from ref. 11.

Values obtained from ref. 3.

ND, not determined.