. Author manuscript; available in PMC: 2014 Jul 1.

Published in final edited form as: Nat Protoc. 2013 Dec 19;9(1):146–155. doi: 10.1038/nprot.2014.001

TABLE 2.

Spinach-based sensor sequences.

Name	Sequence^a
Spinach	GACGCAACTGAATGAAATGGTGAAGGACGGGTCCAGGTGTGGCTGCTTCGGCAGTGCAGCTTGTTGAGTAGAGTGTGAGCTCCGTAACTAGTCGCGTC
ADP sensor	GACGCAACTGAATGAAATGGTGAAGGACGGGTCCAGCACGAGGGGGAAACCCCGGACAATCAGACACGGTGCTTGTTGAGTAGAGTGTGAGCTCCGTAACTAGTCGCGTC

The Spinach module of the sensor (top row) is shown in normal font; underlined is the sequence of its stem-loop 3, which is to be replaced by the molecule-specific module. The molecule-specific module is shown inserted into the Spinach sequence in the bottom row, in bold, and comprises the transducer module (underline) and the molecule recognition module (italics). See Figure 1 for more information.