Table 1.

Oligonucleotide primers for amplification of X. laevis ADHs

Name	Nucleotide sequence 1	Amino acid residues 2
Degenerate primers (based on Rana perezi ADH8)
Degenerate forward outer	5′-ATGTGCACTGCGGGIAARGWIATHA-3′	1-8
Degenerate reverse outer	5′-TAGTCTTTTGGRTTIADRCAYTC-3′	237-244
Degenerate forward inner	5′-ATTACATGTAAGGCIGCIGTIGC-3′	7-14
Degenerate reverse inner	5′-GGCTTTTGGRAAYTTRTCYTTRT-3′	223-230
Specific forward primers (used in combination with 3′-end adaptors)
ADH1B outer	5′-GTATAGTGGAAAGTGTGGGAGAG-3′	72-79
ADH1B inner	5′-CATACATTGGACTCTTGTTGGAC-3′	119-126
ADH3 outer	5′-CTGAATACACTGTTGTAGC-A-3′	148-154
ADH3 inner	5′-CAACTGGTTATGGAGCTGTG-3′	178-184
ADH8B outer	5′-AGCACTTTTACAGAATACAG-3′	144-150
ADH8B inner	5′-AGATTCCTCCAGGATCTACG-3′	186-192
ADH9 outer	5′-GCTAAAGTACAGCAAGGTAG-3′	189-194
ADH9 inner	5′-GAATCATTGGAGTAGACATT-3′	219-225
Specific reverse primers for ADH8B (used in combination with the above specific forward primers)
ADH8B reverse outer	5′-TCAACAGGATGTCAGGCTGCAAATG-3′	Nucleotides 61–37 of non-coding 3′-end
ADH8B reverse inner	5′-AATGACCGTAGTGGACTTCACACGA-3′	Nucleotides 40–16 of non-coding 3′-end

¹Degenerate nucleotides are R (A or G), W (A or T), H (A or C or T) and I (inosine, able to base pair with any natural nucleotide). ²Numbering refers to amino acid residues unless otherwise indicated.