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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Mol Cancer Res. 2013 Aug 8;11(10):1215–1222. doi: 10.1158/1541-7786.MCR-13-0170

Figure 1.

Figure 1

Menin inhibits GLI1 independent of the canonical Hedgehog (Hh) pathway. (A) Menin-null MEFs were complemented with either vector or wild type (WT) menin, and the mRNA levels of Gli1 were determined by qRT-PCR. Data is expressed as fold change over menin-expressing cells. (B) Whole cell lysates from menin-null MEFs complemented with either vector or WT menin were subjected to SDS-PAGE electrophoresis, and the protein levels of menin and PTCH1 were determined by immunoblotting. (C) Menin-null MEFs complemented with either vector or WT menin were cultured in the presence of 100 nM Smoothened Agonist (SAG) and the mRNA levels of Gli1 were determined by qRT-PCR. Data is expressed as fold change over menin-null cells cultured in the absence of SAG. Error bars indicate ± s.d.