Table 2. Details of the F. virguliforme proteins identified in cell-free culture filtrates.

Protein IDa	Description	Protein score	Proteinmass (kDa)	# ofpeptides	Timesidentifiedb	emPAIc	Signalpeptide
g10227d	Lipoprotein_15Secreted repeat ofunknown function	11495	13.06	6	620	24.74	Y
g3913d	Cerato-platanin	2844	15.19	8	266	19.59	Y
g11360d	Unknown	2716	24.96	9	124	6.46	Y
g11634	Unknown	1899	19.99	7	76	3.74	Y
g12110d	PAN_1 PAN domain	1808	28.21	9	118	5.68	Y
g5600	Glycolipid anchoredsurface protein (GAS1)	1169	58.57	9	34	0.93	Y
g13851	Alpha amylase inhibitor	963	17.01	4	48	1.48	Y
g8948d	Unknown	880	20.85	2	23	0.35	Y
g12236	Unknown	774	15.60	8	69	4.85	Y
g11991	Glycosyl hydrolases family 16	673	29.25	7	35	0.91	Y
g7574	Cutinase	672	15.78	2	30	0.79	Y
g8691	TIL Trypsin Inhibitorlike cysteine rich domain	546	8.96	2	16	1.68	Y
g2004	Glycosyl hydrolasesfamily 15	543	78.02	8	26	0.51	Y
g6599	Unknown	506	15.87	3	12	1.17	Y
g4858	CFEM domain	432	23.13	3	30	1.58	N
g1353	Sporozoite P67surface antigen	419	21.89	2	9	0.33	N
g9768	Unknown	366	38.18	3	8	0.39	Y
g10536	CFEM domain	360	94.64	2	13	0.15	Y
g6924	FvTox1	346	40.75	2	20	0.37	Y
g13548	Glycosyl hydrolase family 4	312	40.11	4	32	0.17	Y
g2624	Catalase	263	78.29	3	15	0.23	Y
g11346	PLA2_B Lysophospholipasecatalytic domain	259	72.69	5	9	0.14	Y
g3867	Glycolipid anchoredsurface protein (GAS1)	252	49.54	3	6	0.21	Y
g10551	Glycosylhydrolases family 16	235	46.11	4	27	0.51	Y
g8790	Unknown	225	15.01	3	10	1.27	Y
g4057	Unknown	218	26.91	6	26	0.6	Y
g12686	Laminin_G_2 LamininG domain	208	29.63	4	9	0.53	Y
g4142	Unknown	207	18.56	3	17	0.95	Y
g7538	Isochorismatase	197	27.15	3	15	0.41	N
g1131	Unknown	189	32.68	2	5	0.21	Y
g3481	Squalene epoxidase	183	86.96	2	7	0.16	N
g7658	Cerato-platanin	164	23.72	2	4	0.3	Y
g2029	Glyceraldehyde 3-phosphatedehydrogenase,C-terminal domain	158	36.21	2	8	0.19	Y
g1554	Beta-glucosidase(SUN family)	136	47.22	2	3	0.14	Y
g7569	Eukaryotic-typecarbonic anhydrase	120	33.89	5	10	0.6	Y
g6537	Cis-muconatelactonizing enzyme	113	40.78	4	8	0.37	Y
g6259	Glycosyl hydrolasefamily 45	101	30.09	2	6	0.11	Y
g8786	Unknown	90	146.50	2	2	0.05	N
g5055	Ubiquitin Ubiquitin family	84	113.49	3	8	0.06	N
g8971	Chitosanase Fungalchitosanase	71	32.54	2	2	0.1	Y
g2888	N terminal extensionof bacteriophageendosialidase	68	87.69	2	3	0.08	Y
g2768	Unknown	51	15.18	2	2	0.5	Y
g8551	Trypan_PARP(Procyclic acidic repetitive protein)	50	69.16	2	5	0.1	Y
g12211	Peptidase family M28	40	38.73	2	2	0.18	N

^aProtein identification numbers are same as the gene IDs of the F. virguliforme genome database (http://fvgbrowse.agron.iastate.edu) [56].

^bNumber of times a peptide(s) was identified in one biological replicate of the culture filtrate.

^cExponentially modified protein abundance index.

^d F. virguliforme proteins that were identified from the xylem sap of F. virguliforme-infected soybean plants (Table 1).