Table 3. Soybean proteins identified from the xylem saps of both F. virguliforme-infected and -uninfected, healthy soybean plants.
Protein IDa | Description | Proteinscore | Proteinmass (kDa) | # ofpeptides | emPAIb | Signalpeptide |
Glyma18g41320.1 | Proteaseinhibitor/seed storage/LTP family | 3281 | 13.0 | 7 | 134.77 | Y |
Glyma08g21410.1 | 50S ribosomal protein | 2005 | 29.4 | 17 | 13.58 | Y |
Glyma07g01730.1 | 50S ribosomal protein | 1684 | 29.2 | 20 | 13.87 | Y |
Glyma06g45700.1 | Glycosyl hydrolase family 14 | 1161 | 55.8 | 20 | 2.17 | N |
Glyma13g34290.1 | Gamma-glutamyl hydrolase | 1073 | 37.8 | 13 | 3.52 | Y |
Glyma02g07140.1 | Ribonuclease T2 family | 630 | 27.0 | 8 | 2.6 | Y |
Glyma05g22180.1 | Peroxidase | 571 | 35.9 | 12 | 2.75 | Y |
Glyma08g45490.1 | Trypsin andprotease inhibitor | 506 | 22.0 | 10 | 5.33 | Y |
Glyma07g17000.2 | Protease inhibitor/seedstorage/LTP family | 492 | 11.1 | 6 | 4.07 | Y |
Glyma16g04240.1 | Methionine synthase II(Cobalamine- independent) | 480 | 84.4 | 23 | 0.84 | N |
Glyma17g17730.1 | Peroxidase | 479 | 36.0 | 11 | 2.75 | Y |
Glyma03g16620.3 | Protease inhibitor/seed storage/LTP family | 459 | 13.9 | 7 | 10.22 | Y |
Glyma12g06910.1 | Heat shockprotein 70 kDa | 445 | 75.6 | 11 | 0.67 | N |
Glyma12g19520.1 | Malate dehydrogenase | 389 | 36.4 | 11 | 1.01 | N |
Glyma03g32850.1 | 70 kDa heatshock protein | 357 | 71.9 | 11 | 0.64 | N |
Glyma04g14650.1 | Acyl CoAbinding protein | 348 | 10.1 | 5 | 9.6 | N |
Glyma06g15030.1 | Peroxidase | 323 | 35.0 | 8 | 0.89 | Y |
Glyma16g04770.1 | Nucleoside phosphatase | 305 | 50.8 | 9 | 0.55 | Y |
Glyma19g37520.1 | Enolase | 305 | 48.0 | 12 | 0.82 | N |
Glyma02g00810.1 | Cytosolic malatedehydrogenase | 297 | 35.9 | 6 | 1.03 | N |
Glyma09g34770.1 | Acyl CoA binding protein | 293 | 10.1 | 5 | 9.6 | N |
Glyma18g52610.1 | Cu/Zn superoxide dismutase | 281 | 65.6 | 17 | 0.47 | N |
Glyma10g40720.1 | Plant Basic Secretory Protein | 280 | 26.9 | 6 | 0.79 | Y |
Glyma19g30140.1 | Calmodulin | 273 | 15.6 | 3 | 0.8 | N |
Glyma17g11790.1 | Purple acidphosphatase-like protein | 260 | 59.7 | 4 | 0.31 | N |
Glyma18g41590.1 | Proteaseinhibitor/seed storage/LTP family | 258 | 11.1 | 3 | 1.95 | Y |
Glyma05g28490.1 | Glycine/serine hydroxymethyltransferase | 256 | 53.3 | 8 | 0.43 | N |
Glyma08g11480.1 | S-adenosylhomocysteine hydrolase | 248 | 53.8 | 9 | 0.35 | N |
Glyma08g18760.1 | TCP-1/cpn60 chaperonin family | 248 | 53.8 | 7 | 0.35 | N |
Glyma03g04960.1 | Proteaseinhibitor/seed storage/LTP family | 239 | 12.9 | 6 | 4.21 | Y |
Glyma11g14950.1 | 70 kD heat shock protein | 230 | 23.4 | 4 | 0.95 | N |
Glyma04g39475.1 | Alginate lyase | 228 | 24.6 | 5 | 0.66 | Y |
Glyma12g06606.1 | ribulose-bisphosphatecarboxylase large chain | 216 | 50.9 | 23 | 0.47 | N |
Glyma17g35720.1 | Cysteine proteinase Cathepsin L | 214 | 52.6 | 3 | 0.2 | Y |
Glyma13g16590.1 | Peroxidase | 213 | 36.1 | 8 | 1.02 | Y |
Glyma07g13710.1 | Nucleosidediphosphate kinase | 211 | 16.5 | 5 | 1.11 | N |
Glyma17g03350.1 | Pathogenesis-relatedprotein Bet v I family | 204 | 16.8 | 3 | 0.74 | N |
Glyma07g39120.1 | Lactoylglutathione Lyase | 196 | 31.7 | 2 | 0.1 | N |
Glyma02g09780.2 | Proteaseinhibitor/seed storage/LTP family | 195 | 10.4 | 5 | 4.58 | Y |
Glyma12g07780.3 | Peroxidase | 193 | 27.2 | 5 | 0.78 | N |
Glyma06g10650.2 | Tyrosine3-monooxygenase/tryptophan 5-monooxygenaseactivation protein | 192 | 29.3 | 8 | 0.91 | N |
Glyma08g45531.1 | Trypsin and proteaseinhibitor | 187 | 24.4 | 6 | 1.17 | N |
Glyma06g28890.1 | Peroxidase | 187 | 35.6 | 5 | 0.31 | Y |
Glyma15g19580.1 | Cysteine proteinaseCathepsin L | 178 | 39.7 | 4 | 0.38 | Y |
Glyma10g43990.1 | Transketolase precursor | 175 | 18.5 | 4 | 0.96 | N |
Glyma11g10240.1 | Pollen allergen | 175 | 52.1 | 4 | 0.42 | Y |
Glyma08g19180.1 | Peroxidase | 170 | 35.1 | 9 | 0.57 | Y |
Glyma05g38130.1 | Thaumatin family | 166 | 22.4 | 7 | 2.06 | N |
Glyma13g41960.1 | Fructokinase | 164 | 18.6 | 2 | 0.4 | N |
Glyma12g07370.1 | Fasciclin domain | 160 | 30.7 | 5 | 0.51 | Y |
Glyma16g08040.1 | Zinc-binding oxidoreductase | 158 | 34.6 | 8 | 0.9 | N |
Glyma14g35530.1 | Plastocyanin-like domain | 153 | 21.2 | 3 | 0.55 | Y |
Glyma07g00900.1 | Lipoxygenase | 151 | 96.9 | 9 | 0.26 | N |
Glyma01g32750.1 | Proteaseinhibitor/seed storage/LTP family | 144 | 12.2 | 3 | 2.45 | Y |
Glyma07g04960.1 | Subtilisin/Kexin-RelatedSerine Protease | 142 | 82.3 | 2 | 0.08 | Y |
Glyma04g04730.1 | Subtilisin/Kexin-Related Serine Protease | 142 | 82.3 | 3 | 0.08 | Y |
Glyma11g10480.2 | Cyclophilin | 141 | 18.4 | 5 | 0.57 | N |
Glyma06g47510.2 | Ribosomal protein L16 | 138 | 21.0 | 2 | 0.35 | N |
Glyma10g13450.1 | Lectin | 134 | 30.2 | 6 | 0.69 | Y |
Glyma18g53150.1 | Proteaseinhibitor/seed storage/LTP family | 134 | 9.4 | 2 | 0.87 | Y |
Glyma04g02240.1 | Plastocyanin | 130 | 16.6 | 4 | 1.52 | N |
Glyma05g37490.1 | Glycosylhydrolases family 28 | 128 | 26.7 | 3 | 0.6 | N |
Glyma05g35970.1 | Profilin | 125 | 14.1 | 2 | 0.24 | N |
Glyma03g40110.1 | 40S ribosomal protein S14 | 122 | 16.4 | 3 | 0.37 | N |
Glyma05g30380.1 | Plastocyanin-like domain | 115 | 13.2 | 4 | 2.98 | Y |
Glyma02g07150.1 | Ribonuclease T2 family | 115 | 27.1 | 7 | 2.19 | Y |
Glyma13g42330.1 | Lipoxygenase | 113 | 69.0 | 9 | 0.44 | N |
Glyma16g00410.1 | Trypsin and protease inhibitor | 112 | 56.0 | 4 | 0.12 | N |
Glyma12g08310.1 | Mitochondrialchaperonin | 110 | 52.9 | 6 | 0.17 | N |
Glyma10g07820.1 | Monodehydroascorbate/ferredoxinreductase | 109 | 43.7 | 5 | 0.24 | N |
Glyma02g05350.1 | ferredoxin–NADP+ reductase [ | 109 | 40.6 | 3 | 0.17 | N |
Glyma04g14640.4 | 60S ribosomalprotein L13 | 108 | 23.8 | 2 | 0.14 | N |
Glyma13g17421.1 | Glycosyltransferase | 105 | 79.1 | 10 | 0.24 | N |
Glyma12g02790.2 | Cyclophilin | 104 | 18.4 | 2 | 0.66 | N |
Glyma08g41280.1 | 60S ribosomalprotein L34 | 102 | 13.8 | 2 | 0.56 | N |
Glyma19g42890.1 | Hsp70 protein | 102 | 62.0 | 4 | 0.14 | N |
Glyma06g02481.1 | Subtilisin/Kexin-Related Serine Protease | 100 | 82.2 | 6 | 0.17 | Y |
Glyma12g16340.1 | Plastocyanin-likedomain | 99 | 22.6 | 2 | 0.32 | Y |
Glyma15g04670.1 | 60S ribosomal protein L23A | 98 | 17.3 | 2 | 0.71 | N |
Glyma15g04805.1 | Histone H4 | 97 | 11.3 | 3 | 1.22 | N |
Glyma17g06090.1 | Peroxidase | 95 | 36.4 | 6 | 0.55 | Y |
Glyma02g18090.1 | Lectin | 94 | 22.6 | 6 | 0.74 | Y |
Glyma05g14330.2 | Proteasomesubunit | 93 | 24.2 | 4 | 0.22 | N |
Glyma04g01020.1 | Fructose-biphosphatealdolase | 92 | 14.1 | 2 | 0.54 | N |
Glyma16g17190.1 | Pectin acetylesterase | 91 | 43.9 | 2 | 0.16 | Y |
Glyma15g09530.1 | GDSL-like Lipase/Acylhydrolase | 91 | 43.1 | 8 | 0.81 | Y |
Glyma06g03410.1 | Nad DependentEpimerase/Dehydratase | 89 | 34.3 | 7 | 0.74 | N |
Glyma17g17850.1 | aspartateaminotransferase | 87 | 53.0 | 5 | 0.19 | N |
Glyma08g43690.1 | 40S ribosomal protein S8 | 86 | 25.1 | 3 | 0.28 | N |
Glyma10g28890.2 | Calreticulin | 81 | 48.3 | 5 | 0.3 | Y |
Glyma14g36850.1 | Fructose-biphosphate aldolase | 81 | 38.5 | 9 | 0.78 | N |
Glyma02g05370.1 | 40S ribosomal protein S4 | 73 | 29.8 | 4 | 0.37 | N |
Glyma08g21390.1 | 60S ribosomal protein L10 | 72 | 29.0 | 2 | 0.24 | Y |
Glyma11g21001.1 | Dirigent-like protein | 71 | 21.2 | 4 | 0.34 | Y |
Glyma11g13580.1 | fructokinase | 71 | 35.5 | 2 | 0.09 | N |
Glyma11g38220.1 | PLAT/LH2 family protein | 70 | 20.4 | 6 | 0.16 | Y |
Glyma16g01090.1 | Subtilisin/Kexin-Related Serine Protease | 70 | 86.6 | 2 | 0.08 | N |
Glyma14g10456.2 | Glycosyl hydrolase family 10 | 70 | 109.9 | 3 | 0.12 | N |
Glyma17g33050.1 | Demethylmenaquinone methyltransferase | 70 | 59.9 | 5 | 0.08 | Y |
Glyma16g33710.1 | Trypsin andprotease inhibitor | 69 | 23.9 | 4 | 0.48 | Y |
Glyma13g39600.1 | Serine carboxypeptidase | 67 | 51.2 | 7 | 0.45 | Y |
Glyma03g23890.1 | Zinc-binding dehydrogenase | 67 | 38.1 | 6 | 0.09 | N |
Glyma06g18110.1 | Glyceraldehyde 3-phosphate dehydrogenase | 65 | 36.8 | 13 | 1.58 | N |
Glyma13g19830.1 | GlutathioneS-transferase | 65 | 27.1 | 2 | 0.26 | N |
Glyma12g31850.3 | Carboxymethylenebutenolidase | 63 | 21.9 | 2 | 0.23 | N |
Glyma18g47760.1 | Hsp70 protein | 58 | 28.9 | 2 | 0.17 | N |
Glyma08g45610.1 | Trypsin andprotease inhibitor | 52 | 26.2 | 3 | 0.43 | Y |
Glyma03g26060.1 | Plastocyanin-likedomain | 45 | 19.3 | 3 | 0.62 | Y |
Glyma20g26610.1 | Plant basicsecretory protein | 44 | 25.3 | 9 | 1.7 | Y |
Glyma18g44810.1 | Cellulase(glycosyl hydrolase family 5) | 42 | 52.8 | 5 | 0.27 | Y |
Glyma03g22260.1 | Protein ofunknown function(DUF568) | 38 | 25.7 | 2 | 0.28 | Y |
Glyma15g04290.1 | Triosephosphateisomerase | 33 | 27.4 | 7 | 0.77 | N |
Glycine max protein identification number from Phytozome database (http://www.phytozome.net/search.php).
Exponentially modified protein abundance index.