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. 2014 May 20;9(5):e93667. doi: 10.1371/journal.pone.0093667

Table 3. Soybean proteins identified from the xylem saps of both F. virguliforme-infected and -uninfected, healthy soybean plants.

Protein IDa Description Proteinscore Proteinmass (kDa) # ofpeptides emPAIb Signalpeptide
Glyma18g41320.1 Proteaseinhibitor/seed storage/LTP family 3281 13.0 7 134.77 Y
Glyma08g21410.1 50S ribosomal protein 2005 29.4 17 13.58 Y
Glyma07g01730.1 50S ribosomal protein 1684 29.2 20 13.87 Y
Glyma06g45700.1 Glycosyl hydrolase family 14 1161 55.8 20 2.17 N
Glyma13g34290.1 Gamma-glutamyl hydrolase 1073 37.8 13 3.52 Y
Glyma02g07140.1 Ribonuclease T2 family 630 27.0 8 2.6 Y
Glyma05g22180.1 Peroxidase 571 35.9 12 2.75 Y
Glyma08g45490.1 Trypsin andprotease inhibitor 506 22.0 10 5.33 Y
Glyma07g17000.2 Protease inhibitor/seedstorage/LTP family 492 11.1 6 4.07 Y
Glyma16g04240.1 Methionine synthase II(Cobalamine- independent) 480 84.4 23 0.84 N
Glyma17g17730.1 Peroxidase 479 36.0 11 2.75 Y
Glyma03g16620.3 Protease inhibitor/seed storage/LTP family 459 13.9 7 10.22 Y
Glyma12g06910.1 Heat shockprotein 70 kDa 445 75.6 11 0.67 N
Glyma12g19520.1 Malate dehydrogenase 389 36.4 11 1.01 N
Glyma03g32850.1 70 kDa heatshock protein 357 71.9 11 0.64 N
Glyma04g14650.1 Acyl CoAbinding protein 348 10.1 5 9.6 N
Glyma06g15030.1 Peroxidase 323 35.0 8 0.89 Y
Glyma16g04770.1 Nucleoside phosphatase 305 50.8 9 0.55 Y
Glyma19g37520.1 Enolase 305 48.0 12 0.82 N
Glyma02g00810.1 Cytosolic malatedehydrogenase 297 35.9 6 1.03 N
Glyma09g34770.1 Acyl CoA binding protein 293 10.1 5 9.6 N
Glyma18g52610.1 Cu/Zn superoxide dismutase 281 65.6 17 0.47 N
Glyma10g40720.1 Plant Basic Secretory Protein 280 26.9 6 0.79 Y
Glyma19g30140.1 Calmodulin 273 15.6 3 0.8 N
Glyma17g11790.1 Purple acidphosphatase-like protein 260 59.7 4 0.31 N
Glyma18g41590.1 Proteaseinhibitor/seed storage/LTP family 258 11.1 3 1.95 Y
Glyma05g28490.1 Glycine/serine hydroxymethyltransferase 256 53.3 8 0.43 N
Glyma08g11480.1 S-adenosylhomocysteine hydrolase 248 53.8 9 0.35 N
Glyma08g18760.1 TCP-1/cpn60 chaperonin family 248 53.8 7 0.35 N
Glyma03g04960.1 Proteaseinhibitor/seed storage/LTP family 239 12.9 6 4.21 Y
Glyma11g14950.1 70 kD heat shock protein 230 23.4 4 0.95 N
Glyma04g39475.1 Alginate lyase 228 24.6 5 0.66 Y
Glyma12g06606.1 ribulose-bisphosphatecarboxylase large chain 216 50.9 23 0.47 N
Glyma17g35720.1 Cysteine proteinase Cathepsin L 214 52.6 3 0.2 Y
Glyma13g16590.1 Peroxidase 213 36.1 8 1.02 Y
Glyma07g13710.1 Nucleosidediphosphate kinase 211 16.5 5 1.11 N
Glyma17g03350.1 Pathogenesis-relatedprotein Bet v I family 204 16.8 3 0.74 N
Glyma07g39120.1 Lactoylglutathione Lyase 196 31.7 2 0.1 N
Glyma02g09780.2 Proteaseinhibitor/seed storage/LTP family 195 10.4 5 4.58 Y
Glyma12g07780.3 Peroxidase 193 27.2 5 0.78 N
Glyma06g10650.2 Tyrosine3-monooxygenase/tryptophan 5-monooxygenaseactivation protein 192 29.3 8 0.91 N
Glyma08g45531.1 Trypsin and proteaseinhibitor 187 24.4 6 1.17 N
Glyma06g28890.1 Peroxidase 187 35.6 5 0.31 Y
Glyma15g19580.1 Cysteine proteinaseCathepsin L 178 39.7 4 0.38 Y
Glyma10g43990.1 Transketolase precursor 175 18.5 4 0.96 N
Glyma11g10240.1 Pollen allergen 175 52.1 4 0.42 Y
Glyma08g19180.1 Peroxidase 170 35.1 9 0.57 Y
Glyma05g38130.1 Thaumatin family 166 22.4 7 2.06 N
Glyma13g41960.1 Fructokinase 164 18.6 2 0.4 N
Glyma12g07370.1 Fasciclin domain 160 30.7 5 0.51 Y
Glyma16g08040.1 Zinc-binding oxidoreductase 158 34.6 8 0.9 N
Glyma14g35530.1 Plastocyanin-like domain 153 21.2 3 0.55 Y
Glyma07g00900.1 Lipoxygenase 151 96.9 9 0.26 N
Glyma01g32750.1 Proteaseinhibitor/seed storage/LTP family 144 12.2 3 2.45 Y
Glyma07g04960.1 Subtilisin/Kexin-RelatedSerine Protease 142 82.3 2 0.08 Y
Glyma04g04730.1 Subtilisin/Kexin-Related Serine Protease 142 82.3 3 0.08 Y
Glyma11g10480.2 Cyclophilin 141 18.4 5 0.57 N
Glyma06g47510.2 Ribosomal protein L16 138 21.0 2 0.35 N
Glyma10g13450.1 Lectin 134 30.2 6 0.69 Y
Glyma18g53150.1 Proteaseinhibitor/seed storage/LTP family 134 9.4 2 0.87 Y
Glyma04g02240.1 Plastocyanin 130 16.6 4 1.52 N
Glyma05g37490.1 Glycosylhydrolases family 28 128 26.7 3 0.6 N
Glyma05g35970.1 Profilin 125 14.1 2 0.24 N
Glyma03g40110.1 40S ribosomal protein S14 122 16.4 3 0.37 N
Glyma05g30380.1 Plastocyanin-like domain 115 13.2 4 2.98 Y
Glyma02g07150.1 Ribonuclease T2 family 115 27.1 7 2.19 Y
Glyma13g42330.1 Lipoxygenase 113 69.0 9 0.44 N
Glyma16g00410.1 Trypsin and protease inhibitor 112 56.0 4 0.12 N
Glyma12g08310.1 Mitochondrialchaperonin 110 52.9 6 0.17 N
Glyma10g07820.1 Monodehydroascorbate/ferredoxinreductase 109 43.7 5 0.24 N
Glyma02g05350.1 ferredoxin–NADP+ reductase [ 109 40.6 3 0.17 N
Glyma04g14640.4 60S ribosomalprotein L13 108 23.8 2 0.14 N
Glyma13g17421.1 Glycosyltransferase 105 79.1 10 0.24 N
Glyma12g02790.2 Cyclophilin 104 18.4 2 0.66 N
Glyma08g41280.1 60S ribosomalprotein L34 102 13.8 2 0.56 N
Glyma19g42890.1 Hsp70 protein 102 62.0 4 0.14 N
Glyma06g02481.1 Subtilisin/Kexin-Related Serine Protease 100 82.2 6 0.17 Y
Glyma12g16340.1 Plastocyanin-likedomain 99 22.6 2 0.32 Y
Glyma15g04670.1 60S ribosomal protein L23A 98 17.3 2 0.71 N
Glyma15g04805.1 Histone H4 97 11.3 3 1.22 N
Glyma17g06090.1 Peroxidase 95 36.4 6 0.55 Y
Glyma02g18090.1 Lectin 94 22.6 6 0.74 Y
Glyma05g14330.2 Proteasomesubunit 93 24.2 4 0.22 N
Glyma04g01020.1 Fructose-biphosphatealdolase 92 14.1 2 0.54 N
Glyma16g17190.1 Pectin acetylesterase 91 43.9 2 0.16 Y
Glyma15g09530.1 GDSL-like Lipase/Acylhydrolase 91 43.1 8 0.81 Y
Glyma06g03410.1 Nad DependentEpimerase/Dehydratase 89 34.3 7 0.74 N
Glyma17g17850.1 aspartateaminotransferase 87 53.0 5 0.19 N
Glyma08g43690.1 40S ribosomal protein S8 86 25.1 3 0.28 N
Glyma10g28890.2 Calreticulin 81 48.3 5 0.3 Y
Glyma14g36850.1 Fructose-biphosphate aldolase 81 38.5 9 0.78 N
Glyma02g05370.1 40S ribosomal protein S4 73 29.8 4 0.37 N
Glyma08g21390.1 60S ribosomal protein L10 72 29.0 2 0.24 Y
Glyma11g21001.1 Dirigent-like protein 71 21.2 4 0.34 Y
Glyma11g13580.1 fructokinase 71 35.5 2 0.09 N
Glyma11g38220.1 PLAT/LH2 family protein 70 20.4 6 0.16 Y
Glyma16g01090.1 Subtilisin/Kexin-Related Serine Protease 70 86.6 2 0.08 N
Glyma14g10456.2 Glycosyl hydrolase family 10 70 109.9 3 0.12 N
Glyma17g33050.1 Demethylmenaquinone methyltransferase 70 59.9 5 0.08 Y
Glyma16g33710.1 Trypsin andprotease inhibitor 69 23.9 4 0.48 Y
Glyma13g39600.1 Serine carboxypeptidase 67 51.2 7 0.45 Y
Glyma03g23890.1 Zinc-binding dehydrogenase 67 38.1 6 0.09 N
Glyma06g18110.1 Glyceraldehyde 3-phosphate dehydrogenase 65 36.8 13 1.58 N
Glyma13g19830.1 GlutathioneS-transferase 65 27.1 2 0.26 N
Glyma12g31850.3 Carboxymethylenebutenolidase 63 21.9 2 0.23 N
Glyma18g47760.1 Hsp70 protein 58 28.9 2 0.17 N
Glyma08g45610.1 Trypsin andprotease inhibitor 52 26.2 3 0.43 Y
Glyma03g26060.1 Plastocyanin-likedomain 45 19.3 3 0.62 Y
Glyma20g26610.1 Plant basicsecretory protein 44 25.3 9 1.7 Y
Glyma18g44810.1 Cellulase(glycosyl hydrolase family 5) 42 52.8 5 0.27 Y
Glyma03g22260.1 Protein ofunknown function(DUF568) 38 25.7 2 0.28 Y
Glyma15g04290.1 Triosephosphateisomerase 33 27.4 7 0.77 N
a

Glycine max protein identification number from Phytozome database (http://www.phytozome.net/search.php).

b

Exponentially modified protein abundance index.