Table 5. Antibodies and flow cytometer settings used for antigen detection.
Antigen | Source | Clone | ng/test | Fluorophore | Excitation | Longpass Dichroic Mirror | Bandpass filter |
CD56 | Biolegend | HCD56 | 400 | Brilliant violet 711 | 405 nm | 685LP | 710/50 |
Viability | eBioscience | 1∶1000 | eF780 | 640 nm | 755LP | 780/60 | |
CD3 | BD Horizon | UCHT1 | 5µl | PE-CF594 | 561 nm | 600LP | 610/20 |