Table 6. Antibodies and flow cytometer settings used for antigen detection.

Antigen	Source	Clone	ng/test	Fluorophore	Excitation	Longpass Dichroic Mirror	Bandpass filter
CD4	Biolegend	OKT4	100	Brilliant violet 510	405 nm	505LP	525/50
CD3	Biolegend	OKT3	250	Brilliant violet 605	405 nm	595LP	605/40
FoxP3	BD	259D/C7	500	AF488	488 nm	505LP	530/30
T-bet	Biolegend	4B10	100	PerCP-Cy5.5	488 nm	685LP	695/40
CD8	Biolegend	HIT8a	2500	AF700	640 nm	685LP	710/50
Viability	eBioscience		1∶1000	eF780	640 nm	755LP	780/60