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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Nat Chem Biol. 2014 Apr 28;10(6):450–456. doi: 10.1038/nchembio.1520

Figure 4. BABA interferes with canonical AspRS activity.

Figure 4

(a) Western blot analysis of eIF2α phosphorylation (Ser51) and TCTP (loading control) in ibi1-1, Col-0, gcn2-1 and Ler at 2 days after spraying the leaves with BABA (250 μM). (b) MALDI-qTOF quantification of Asp levels in leaves of Col-0 at 2 days after root treatment with water, R-BABA (250 μM), or S-BABA (250 μM). (c) MALDI-qTOF quantification of Asp levels in leaves of Col-0, ibi1-1 and ibi1-1 35S:IBI1:YFP-1. Data in (b) and (c) represent average fold-change values in comparison to basal Asp levels in water-treated Col-0. Asterisks indicate statistically significant differences in comparison to water-treated Col-0 (Student’s t-test, p<0.05, n=3-5).