Figure 2. Changes of medium temperature during consecutive insertion of 6 vitrified microdrops (denoted with arrows) in a common 35 mm petri dish containing 2.5 ml of warming medium.

A) a typical pattern of temperature changes on a 38°C warmplate; B) a typical pattern of temperature changes on a 42°C warmplate; C) mean ± SEM values of maximum and minimum temperatures recorded on 38°C and 42°C warmplates. Values with different superscripts (a, b) between treatment groups differ significantly (P<0.05) by student’s t-test.