Table 3. Distribution of intestinal parasites (microscopy and/or PCR) and genetic characterization of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi among children from the two communities analyzed.
Agostinho Neto Village (N = 134) | Aires de Menezes Hospital (N = 214) | |||||
Microscopy n (%) | PCR n (%) | Species typinga (n) | Microscopy n (%) | PCR n (%) | Species typinga (n) | |
Helminthes | ||||||
Ascaris lumbricoides | 37 (27.6) | ND | ND | 22 (10.3) | ND | ND |
Ancylostoma spp. | 1 (0.8) | ND | ND | 1 (0.5) | ND | ND |
Hymenolepis nana | 2 (1.5) | ND | ND | 0 | ND | ND |
Hymenolepis sp. | 3 (2.2) | ND | ND | 0 | ND | ND |
Schistosoma intercalatum | 0 | ND | ND | 2 (0.9) | ND | ND |
Strongyloides stercoralis | 1 (0.8) | ND | ND | 0 | ND | ND |
Trichuris trichiura | 6 (4.5) | ND | ND | 14 (6.5) | ND | ND |
Protozoa | ||||||
Cryptosporidium spp. | 0 | 0 | 0 | 9 (4.2) | 19 (8.9) | |
C. hominis | ND | 0 | 0 | ND | 14 (6.5) | IeA11G3T3R1 (3) IeA11G3T3 (4) IaA27R3 (5) IaA23R3 (2) |
C. parvum | ND | 0 | 0 | ND | 5 (2.3) | IIdA21G1a(1) IIdA26G1 (2) IIaA16G2R1(1) IIaA15G2R1(1) |
Cyclospora sp. | 1 (0.8) | ND | 0 | ND | ||
Entamoeba coli | 14 (10.5) | ND | 1 (0.5) | ND | ||
Entamoeba sp. | 2 (1.5) | ND | 0 | ND | ||
Giardia duodenalis | 25 (7.5) | 10 (7.5)b | Ac (6); B (4) | 4 (1.9) | 1 (0.5)b | B (1) |
Fungi | ||||||
Enterocytozoon bieneusi | ND | 7 (5.2) | Type IV(4); Kin1 (2); Kin3 (1) | ND | 19 (8.9) | Type IV (10), D (5), A (2), Kin1 (2) |
Note: aSubtypes, Assemblages and genotypes are indicated for C. hominis and C. parvum, G. duodenalis and E. bieneusi, respectively; bSamples identified by PCR were also positive by microscopy; cDespite several efforts is was not possible to identified A sub-assemblages; ND – Not determined.