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. 2014 May 20;9(5):e97880. doi: 10.1371/journal.pone.0097880

Figure 5. Effects of luteolin on 6-OHDA-mediated GRP78 and CHOP expression in PC12 cells.

Figure 5

(A and C) PC12 cells were cultured in serum-free medium and then incubated with 6-OHDA (100 µM) for 8 and 12 h. Cells treated with tunicamycin (Tm, 1 µg/ml) for 8 h served as a positive control. Levels of GRP78 and CHOP mRNA were measured by RT-Q-PCR and normalized to β-actin as described in the Materials and Methods. (B, D, E and F) PC12 cells were treated with luteolin or tiron for 30 min before 6-OHDA (100 µM) insult for 8 h. RNA was then prepared for RT-Q-PCR analysis. Data represent the mean ± SD of three independent experiments. **, p<0.01 represents significant differences compared with the vehicle control (without 6-OHDA). ##, p<0.01 represents significant differences compared with the 6-OHDA-treated vehicle.