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. 2014 May 20;9(5):e98079. doi: 10.1371/journal.pone.0098079

Figure 4. Δ9-THC reduces METH-induced increase of nNOS neurons in the CPu.

Figure 4

A. Rats received injections of 1 or 3/kg of Δ9-THC either 0.5 h before each METH injection (PRE) or 0.5, 12, 24, 36, and 48 h after the last METH administration (POST), and were sacrificed 3 days after the last METH injection. Pre- and Post-treatment with both doses of Δ9-THC significantly decreased the number of nNOS positive neurons in the CPu. *p<0.05 and **p<0.01 vs PRE METH-VEH; # p<0.05 and ### p<0.001 vs POST METH-VEH (Bonferroni's post-hoc test). Horizontal dot lines represent the values of nNOS positive neurons (31±1.03) in SAL-VEH group. B. Representative images of nNOS immunohistochemical staining 72 h after the last METH or SAL administration in SAL-VEH, METH-VEH, METH-Δ9-THC 1 and 3 mg. Scale bar  =  100 µm.