Figure 4. NbSEC14 affects phospholipids contents.

Control (PVX; gray box) and NbSEC14-silenced (PVX:Sec14P1; black box) leaves were pre-labeled with ³²Pi for 12 h and inoculated with Rs8107. (a) Quantification of DAG and PA in control and NbSEC14-silenced leaves after inoculation with Rs8107. Relative amount of phospholipid was calculated as relative to the none-treated sample. (b) Quantification of DAG and PA in N. benthamiana inoculated with NbSEC14 (NbSEC14) and GUS control (GUS) expressing Agrobacterium. Values represent the means and SD of the results from triplicate experiments. Phospholipid levels are calculated relative to the absolute non-treated control. Asterisks denote values significantly different from GUS-expressing controls (*; P<0.05). Total lipid fractions were separated by ethylacetate TLC for quantification of PA. DAG levels were estimated with DAG kinase system as described in the Materials and Methods.