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. 2014 May 20;9(5):e98150. doi: 10.1371/journal.pone.0098150

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© 2014 Kiba et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) Effect of NbSEC14-silencing on activities of DGK, PI-PLC and PC-PLD. Control (PVX; gray box) and NbSEC14-silenced (PVX:Sec14P1; black box) N. benthamiana leaves were inoculated with Rs8107 and incubated at 25°C for indicated times. Phospholipid quantity was calculated relative to the absolute PVX at time 0. (b) DGK and phospholipase activity in NbSEC14-expressed plants. Total protein extracts were prepared from N. benthamiana leaves inoculated with NbSEC14 (NbSEC14) and GUS control (GUS) expressing Agrobacterium. Phospholipid levels are calculated relative to the non-treated control samples. Values represent the means and SD of the results from triplicate experiments. Asterisks denote values significantly different from controls (0) (*; P<0.05).