Figure 6. RT-PCR and western blot analysis of Ulk1/2 DKO and Atg5 KO lungs. RT-PCR analysis of genes encoding (A) surfactant proteins SFTPA, SFTPB, SFTPC and SFTPD, (B) ABCA3, FASN and NKX2-1 were measured at ED18.5 in Ulk1/2 Ctrl; Ulk1/2 DKO; Atg5 Ctrl and Atg5 KO lung RNA. Data are presented as mean ± SEM where n = 4 for Ulk1/2 Ctrl, n = 5 for Ulk1/2 DKO, n = 3 for Atg5 Ctrl and Atg5 KO respectively for the analysis of SFTPA, SFTPC, SFTPD, and NKX2-1. For analysis of SFTPB, ABCA3, and FASN the n = 6 for Ulk1/2 Ctrl and Ulk1/2 DKO respectively and n = 3 for Atg5 Ctrl and Atg5 KO respectively. (C) Western blot analysis for SFTPB presented as relative expression, normalized by litters, following densitometry of multiple western blots, with n = 11 for Ulk1/2 Ctrl, n = 8 for Ulk1/2 DKO, n = 6 for Atg5 Ctrl and n = 6 for Atg5 KO. * indicates P value < 0.05 determined by ANOVA with Bonferonni’s Multiple Comparison test. A representative blot is shown for SFTPB, SFTPB intermediates detected by the NFlank antibody (SFTPB/proSP-B top, 25-kDa intermediate bottom arrowhead), CTSH, and GAPDH.