Figure 1. PARL-cleaved PINK1 can form cytosolic aggregates. (A and B) Microscopic analysis of HeLa cells transiently expressing PINK1-YFP treated with DMSO, valinomycin (Val) or MG132 for 3 h. Cells were immunostained with anti-TOMM20 (A) and with anti-SQSTM1 (B) antibodies. Line scan plots (taken from the images along the white arrows in (A) are shown in the right panel. Scale bars: 10 μm. (C and D) Immunoblotting of total cell lysate (T), supernatant (S) and pellet (P) fractions after solubilization with 2% Triton X-100 followed by centrifugation. Blue and red arrowheads represent the full-length and PARL-cleaved forms, respectively.