Figure 1. Atg32 undergoes starvation-dependent processing. (A) Cells expressing TAP tagged Atg32 under the control of the GAL1 promoter (KWY100) were cultured in YPG to mid-log phase and shifted to SD-N for the indicated times. TAP-Atg32 was monitored by immunoblotting with an antibody that recognizes PA. The arrowhead here and in the following panels indicates the processed band. (B) Cells expressing GAL1 promoter-driven TAP-Atg32 in wild-type (WT; KWY100), atg1∆ (KWY101), and atg11∆ (KWY104) backgrounds were cultured in YPG to mid-log phase and shifted to SD-N for the indicated times. (C) Cells expressing endogenous promoter-driven TAP-Atg32 in a WT background (KWY139) were cultured in YPL to mid-log phase and shifted to SD-N for the indicated times.